Single live cell TGF-β signalling imaging: Breast cancer cell motility and migration is driven by sub-populations of cells with dynamic TGF-β-Smad3 activity

RB Luwor; D Hakmana; J Iaria; TV Nheu; RJ Simpson; HJ Zhu

Journal article

Single live cell TGF-β signalling imaging: Breast cancer cell motility and migration is driven by sub-populations of cells with dynamic TGF-β-Smad3 activity

RB Luwor, D Hakmana, J Iaria, TV Nheu, RJ Simpson, HJ Zhu

Molecular Cancer | BIOMED CENTRAL LTD | Published : 2015

DOI: 10.1186/s12943-015-0309-1

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Abstract

Background: Metastasis is a process where only a small subset of cells is capable of successfully migrating to and propagating at secondary sites. TGF-β signalling is widely known for its role in cancer metastasis and is associated with cell migration in whole cell populations. Findings: We extend these findings by investigating the role of TGF-β signalling in promoting migration and motility by imaging the signalling activity in live, individual MDA-MB-231 cancer cells utilizing a novel Smad3 Td-Tomato reporter adenovirus. Here we find that not all MDA-MB-231 cancer cells have similar TGF-β mediated Smad3 transcription activity and display at least two distinct migratory populations. Import..

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University of Melbourne Researchers

Rodney Luwor Author

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Awarded by National Science Foundation

Funding Acknowledgements

This work was supported by the National Health & Medical Research Council of Australia for program grant #487922 (RJS), project grants #280913 and #433619 (H-JZ). RBL is a Melbourne Brain Centre Post-Doctoral Research Fellow (Dept of Surgery and Dept of Medicine, Royal Melbourne Hospital, The University of Melbourne) and is supported by the NHMRC Centre for Research Excellence Grant 1001216.